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. 2012 Sep 20;7(9):e45047. doi: 10.1371/journal.pone.0045047

Figure 1. αvβ5 integrin is required for SbS-stimulated cell restitution.

Figure 1

(A) Polarized HCT-8/E11 cell monolayers were pretreated without or with 10 µg/ml of an anti- αv integrin mAb (anti-αv) for 1 h. Cell monolayers were then wounded as described in Methods and incubated for 5 h in the absence (ctrl) or presence of SbS in a medium either containing anti-αv integrin mAb or not. Phase contrast images were acquired at the indicated times. Data shown are from a representative experiment out of 3 performed. Scale bar: 300 µm. (B) HCT-8/E11 cell monolayers were wounded and incubated with SbS. The monolayers were further incubated without (-) or with the following function-blocking anti-mAbs 1 h before wounding and during cell migration: αvintegrin (αv), αvβ3 integrin (αvβ3), αvβ5 integrin (αvβ5), or αvβ6 integrin (αvβ6). Wound closure was determined as described in Methods. Results are expressed as the percentage of cell migration compared to SbS-treated cells without mAbs. Data represent the mean+SD of 5 separate experiments. Rat-anti DPP IV (DPPIV) and mouse anti-α5 subunit (α5) were used as isotypic Abs controls. *** P<0.001.