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. 2012 Sep 20;7(9):e45047. doi: 10.1371/journal.pone.0045047

Figure 4. αvβ5 integrin is required for HCT-8/E11 cell interaction with vitronectin.

Figure 4

(A) Isolated HCT-8/E11 cells were incubated without (Ctrl) or with SbS (SbS) in the absence (-) or presence of either anti-αv or -α5 integrin mAbs. Cells were then seeded on vitronectin (3,15 µg/ml). Cell adhesion was evaluated as described in Methods. Results are expressed as the percentage of cell adhesion compared to untreated cells (Ctrl). Data represent the mean+SD of 3 separate experiments. (B) HCT-8/E11 cells were transfected with anti -αv-integrin siRNAs (SiRNAαv) or scramble oligos (SiRNACtrl) for 48 h. Cells were then plated on vitronectin for 2 h. Cell-ECM adhesion was evaluated as described in Methods. Results are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. ***P<0.001.