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. 2012 Sep 20;7(9):e45047. doi: 10.1371/journal.pone.0045047

Figure 6. SbS blocked αvβ5 integrin interaction with vitronectin.

Figure 6

(A) Vitronectin- or Fibronectin-coated plates were incubated in the absence (Vitronectin and Fibronectin) or presence of SbS (vitronectin+ SbS and fibronectin+ SbS) prior to addition of cells. Isolated HCT-8/E11 cells incubated without (light bars) or with SbS (dark bars) were then seeded on plates. Cell adhesion to ECM was evaluated as described in Methods. Results are expressed as the percentage of cell adhesion compared to untreated cells (Ctrl). Data represent the mean + SD of 3 separate experiments. (B) Isolated HT29-D4 cells were treated with or without SbS (dilution 1/8) and plated on vitronectin at the indicated concentrations. Cell-vitronectin adhesion was evaluated as described in Methods. Results are expressed as the percentage of cell adhesion. Data represent the mean+SD of 3 separate experiments. *** P<0.001.