Table 1. Frequency of splenic B, T, NK, and NKT cell subsets in untreated or MCMV-infected WT or TLR9^−/− male and female mice.

	Uninfected				MCMV-infected
Cell type	WT		TLR9−/−		WT		TLR9−/−
	male	female	male	female	male	female	male	female
B cells	63.1±0.9	60.8±1.6	58.2±3.5	61.8±1.7	63.5±1.4	61.4±4.0	64.6±1.2	63.2±1.2
CD4 T cells	51.3±2.9	49.8±3.7	46.0±7.4	48.3±2.1	51.5±3.6	47.3±1.4	50.3±4.9	44.5±1.4
CD8 T cells	17.0±0.4	17.9±1.3	16.9±1.3	20.7±2.2	18.3±0.6	19.5±3.3	16.4±1.1	21.8±1.8
NK cells	1.9±0.5	2.4±0.5	2.2±0.7	2.0±0.2	1.4±0.2	1.6±0.5	1.5±0.3	2.0±±0.4
NKT cells	1.3±0.1	1.4±0.1	1.1±0.2	1.2±0.1	1.2±0.2	1.0±0.1	1.2±0.2	1.2±0.2

WT and TLR9^−/− male and female mice were left uninfected or infected i.p. with 1,2×10⁵ PFU of MCMV. After, 36 h spleens were harvested, total splenocytes were isolated, stained for B220, CD19, CD3, CD4, CD8, or NK1.1 and analyzed by flow cytometry. The frequency of B cells (CD19⁺B220⁺), CD4 (CD3⁺CD4⁺) and CD8 (CD3⁺CD8⁺) T cells, and NK (B220⁻NK1.1⁺CD3⁻), and NKT (B220⁻NK1.1⁺CD3⁻) cells was determined after gating among live cells. All values denote percentages (mean ± SD) of three mice per group, and are representative of two or three independent experiments.