Table 1. Frequency of splenic B, T, NK, and NKT cell subsets in untreated or MCMV-infected WT or TLR9−/− male and female mice.
Uninfected | MCMV-infected | |||||||
Cell type | WT | TLR9−/− | WT | TLR9−/− | ||||
male | female | male | female | male | female | male | female | |
B cells | 63.1±0.9 | 60.8±1.6 | 58.2±3.5 | 61.8±1.7 | 63.5±1.4 | 61.4±4.0 | 64.6±1.2 | 63.2±1.2 |
CD4 T cells | 51.3±2.9 | 49.8±3.7 | 46.0±7.4 | 48.3±2.1 | 51.5±3.6 | 47.3±1.4 | 50.3±4.9 | 44.5±1.4 |
CD8 T cells | 17.0±0.4 | 17.9±1.3 | 16.9±1.3 | 20.7±2.2 | 18.3±0.6 | 19.5±3.3 | 16.4±1.1 | 21.8±1.8 |
NK cells | 1.9±0.5 | 2.4±0.5 | 2.2±0.7 | 2.0±0.2 | 1.4±0.2 | 1.6±0.5 | 1.5±0.3 | 2.0±±0.4 |
NKT cells | 1.3±0.1 | 1.4±0.1 | 1.1±0.2 | 1.2±0.1 | 1.2±0.2 | 1.0±0.1 | 1.2±0.2 | 1.2±0.2 |
WT and TLR9−/− male and female mice were left uninfected or infected i.p. with 1,2×105 PFU of MCMV. After, 36 h spleens were harvested, total splenocytes were isolated, stained for B220, CD19, CD3, CD4, CD8, or NK1.1 and analyzed by flow cytometry. The frequency of B cells (CD19+B220+), CD4 (CD3+CD4+) and CD8 (CD3+CD8+) T cells, and NK (B220−NK1.1+CD3−), and NKT (B220−NK1.1+CD3−) cells was determined after gating among live cells. All values denote percentages (mean ± SD) of three mice per group, and are representative of two or three independent experiments.