FIG. 3.
ADMSC therapy reduces inflammatory cellular infiltrate and improves insulin and PDX-1 expression in pancreatic islets. A: Histological analysis of the pancreas by H-E and immunohistochemistry for insulin and PDX-1 expression, respectively. Original magnification ×400. B: Quantification of mononuclear cellular infiltrate (or insulitis score) in the pancreatic islets was determined by counting approximately 60 islets/experimental group from at least five mice/group using the following classification: 0 (no cellular infiltration), 0–25, 25–50, >50, and 100% of cell infiltration in each islet. C: The positive area for insulin in the pancreatic islets was quantified using the imaging software NIS-Elements AR 3.2 (Nikon). Data are average of % insulin positive area/squared pixels (px2) ± SEM. *P < 0.05. (A high-quality color representation of this figure is available in the online issue.)