FIG. 5.

Therapy with ADMSCs increases the frequency of Tregs in PLNs of diabetic ADMSC-treated mice. Frequency of CD4⁺CD25⁺Foxp3⁺ T cells was analyzed by flow cytometry in cells obtained from the PLNs. The cells were stained for surface markers CD4 and CD25 and afterward for transcription factor Foxp3. A–C: The frequency of CD4⁺CD25⁺, CD4⁺Foxp3⁺, and CD4⁺CD25⁺Foxp3⁺ T cells in the CD4⁺ cell gate, respectively, for each group (n = 5/group). D–F: Absolute numbers of CD4⁺CD25⁺, CD4⁺Foxp3⁺, and CD4⁺CD25⁺Foxp3⁺ T cells regarding total number of cells in the PLNs were determined for each experimental group. Data are average ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.