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. 2012 Sep 20;8(9):e1002924. doi: 10.1371/journal.pgen.1002924

Figure 4. The R200Q and R227W alleles and proteins do not exhibit dominant behavior.

Figure 4

(A) Wild-type, orJ/+, R200Q/+, and R227W/+ eyes were indistinguishable at P0. No significant differences in eye circumferences were detected. (B–E) Merged images of cryosections showing DAPI staining (blue) and melanogenic pigmentation (white) for each of the indicated genotypes at P0. (F–I) Expression of the retinal ganglion cell marker POU4F2 in wild-type or Vsx2 heterozygous retinas. (J) Eye circumference of orJ/R227W heterozygotes was intermediate to orJ and R227W homozygotes. (K–S) Expression of VSX2, CCND1, and TUBB3 in orJ, orJ/R227W, and R227W retinas at P0. VSX2 was detected in the orJ/R227W retina only. CCND1 and TUBB3 were detected in orJ or orJ/R227W retinas but not in R227W pigmented retina. (T) Genotype-phenotype correlation of Vsx2 alleles arranged by retinal phenotype. * P≤0.05 Scale bars: 1 mm (A, J); 100 µm (B–I, K–S).