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. 2012 Sep 20;8(9):e1002926. doi: 10.1371/journal.pgen.1002926

Figure 1. A genetic screen in mice identifies Cadm1 as a candidate germline modifier of metastasis.

Figure 1

(a) NZB and C58 mice were crossed to FVB mice then re-crossed with MMTV-PyMT-transgenic FVB mice. (b) Primary tumor, pulmonary metastasis, and genotype data was obtained from transgene-positive mice developed tumors and was used to generate QTL maps for pulmonary metastasis. Subcongenic lines containing fragments of NZB chromosome 9 (c) were crossed to MMTV-PyMT transgenic FVB mice and tumor size and pulmonary surface metastases were measured in the progeny (d). No significant change in tumor mass was observed, however a significant increase in metastasis was observed in the N9(49–60) subcongenic line (p = 0.022), resolving the locus of susceptibility was resolved to 49–60 Mb of chromosome 9. (e) Real time quantitative PCR measurement of cDNA from mammary tumor and lung tissue demonstrated a 1.5 fold higher expression of Cadm1 in NZB and NF9 relative to FVB.