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. 2012 Sep 20;8(9):e1002926. doi: 10.1371/journal.pgen.1002926

Figure 2. The effect of Cadm1 over-expression on tumor growth and metastasis in vivo.

Figure 2

(a) Expression of endogenous and exogenous V5-epitope tagged Cadm1 protein (IB, immunoblot) and total protein by ECL densitometry on immunoblot. (d) Total RNA levels of Cadm1 measured by qRT-PCR (mean +/− standard error of the mean [SEM]). Mvt-1 and 6DT1 mouse mammary tumor cells stably expressing Cadm1 were implanted into mammary fat pads of syngeneic FVB mice. Data on primary tumor burden (Mvt-1: p = 0.0031; 6DT1: p = 0.2261) (b), pulmonary surface metastases (c) was collected on day 30 (Mvt-1: p = 0.0026; 6DT1: p = 0.0089; median +/− interquartile range; n = 10 mice per group). (e) Pulmonary surface metastases were normalized by primary tumor mass to determine if Cadm1 overexpression showed an effect on metastasis independent of its potential tumor suppressive activity (Mvt-1: p = 0.0048; 6DT1: p = 0.0185; median +/− interquartile range; n = 10 mice per group). Arrows indicate several but not all visible metastatic nodules present on lung surfaces. (f) Images of whole lungs of two mice implanted with 6DT1 control cells and two mice implanted with 6DT1 cells expressing Cadm1. (g) Results of tail vein injection study (Mvt-1: p = 0.0361; 6DT1: p = 0.0475; median +/− interquartile range; n = 10 mice per group). (N.S., not significant; * defined as p<0.05; ** defined as p<0.01.)