Table 1. AGP Assay Protocol for 1,536-well Format.

Step	Parameter	Value	Description
1	Reagent	3 µL	AGP [4/3x] or buffer
2	Compound(s)	23 or 46 nL	PropranololValidation SetLOPAC1280
3	Time	15 seconds	Centrifugation at 1,000 rpm
4	Time	30 to 60 min	Room Temperature Incubation
5	Reagent	1 µL	Dipyridamole [4x]
6	Time	10 min	Room Temperature Incubation
7	Time	15 seconds	Centrifuge at 1,000 rpm
8	Detection	FP	Envision Read