Table 2. HSA Assay Protocol for 1,536-well Format.
Step | Parameter | Value | Description |
1 | ReagentReagent | 3 µL3 µL | 10 µM HSA/3 µM Dansyl sarcosine3 µM Dansyl sarcosine |
2 | Compound(s) | 23 or 46 nL | NaproxenPhenylbutazoneValidation SetLOPAC1280 |
3 | Time | 15 seconds | Centrifugation at 1,000 rpm |
4 | Time | 10 min | Room Temperature Incubation |
5 | Detection | FP | ViewLux Read |