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. 2012 Sep 20;7(9):e45594. doi: 10.1371/journal.pone.0045594

Table 2. HSA Assay Protocol for 1,536-well Format.

Step Parameter Value Description
1 ReagentReagent 3 µL3 µL 10 µM HSA/3 µM Dansyl sarcosine3 µM Dansyl sarcosine
2 Compound(s) 23 or 46 nL NaproxenPhenylbutazoneValidation SetLOPAC1280
3 Time 15 seconds Centrifugation at 1,000 rpm
4 Time 10 min Room Temperature Incubation
5 Detection FP ViewLux Read