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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Jul 19;32(9):2232–2240. doi: 10.1161/ATVBAHA.112.254680

Figure 3.

Figure 3

Reversal of the inhibitory effect of PI3K/Akt inhibitors on platelet spreading by GSK-3β inhibitor. (A) WT mouse platelets were preincubated with AktX (5 μM), LY294002 (20 μM), or DMSO control in the presence and absence of GSK-3β inhibitor SB216763 (10 μM) and plated on immobilized fibrinogen for 2 hours. Adherent platelets were fixed, permeabilized, stained, and observed with a fluorescence microscope. (B) The surface area of single platelets was quantitated (mean ± SE). Statistical significance was analyzed using Student’s t test. Shown in figure are representative pictures from one of three experiments. Numbers of platelets analyzed are indicated above the bars.