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. Author manuscript; available in PMC: 2013 Jul 17.
Published in final edited form as: Biochemistry. 2012 Jul 3;51(28):5580–5588. doi: 10.1021/bi300672s

Fig. 6.

Fig. 6

Time courses of increases in fluorescence intensity upon binding to VLDL (A) and HDL3 (B) for apoE4 S94C-pyrene (trace a) and W264C-pyrene (trace b). VLDL or HDL3 was added to apoE4 variants at final concentrations of 10 µg/ml apoE4 and 0.1–0.4 mg/ml PL. Pyrene fluorescence was monitored at 385 nm with an excitation of 342 nm. (C) Increases in fluorescence intensity of apoE4 S94C-pyrene (Δ), W264C-pyrene (○), and S290C-pyrene (●) upon binding to HDL3 as a function of the weight ratio of PL to apoE4. Protein concentration was 25 µg/ml.