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Kinetic constants for hydrolysis of GB, GD and GFa.
| Enzyme | Substrate | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|
| Wild-type | GB | 430 ± 50 | 1800 ± 400 | 2.4 (0.6) × 105 |
| Wild-type | GD | 12 ± 1 | 800 ± 200 | 1.5 (0.4) × 104 |
| Wild-type | GF | 210 ± 30 | 900 ± 300 | 2.3 (0.8) × 105 |
| YT | GB | 520 ± 30 | 260 ± 50 | 2.0 (0.4) × 106 |
| YT | GD | 240 ± 20 | 460 ± 90 | 5 (1) × 105 |
| YT | GF | 130 ± 10 | 170 ± 50 | 8 (2) × 105 |
| YTRN | GD | 100 ± 10 | 300 ± 100 | 4 (2) × 105 |
Racemic mixtures of GB, GD, and GF were used for these measurements.
