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77 K resonance Raman spectrum of anaerobically isolated IscR (47% occupancy, ~3 mM), obtained with laser excitation at 488 nm and a power of 100 mW at the sample (resolution is ~6 cm-1). The average of nine scans is presented here, and the contributions from ice lattice vibrations were removed by subtracting a properly scaled spectrum of buffer obtained under identical conditions.
