Table 2.

Tabulated UV/Vis absorbance data for azole fragment binding CYP3A4 and CYP2C9.

	Soret (nm)	α (nm)	β (nm)	α:β	Difference: Peak (nm)	Difference: Trough (nm)	Absmax (1μM)#	Abs390 (1μM)%	KD (mM)
CYP3A4*	416	565	534	0.94	---	---	---	---	---
1,2,3-TRZ	422	565	538	0.77	430	394	0.058	−0.023	14.3 +/− 0.5
1,2,4-TRZ	422	569	537	0.74	432	395	0.0065 0.050	−0.025	KD1=0.0390±0.0071 KD2 = 4.80 ± 0.22
IMZ	424	570	538	0.73	432	407	0.065	−0.027	0.381 +/− 0.011
CYP2C9*	418	569	537	1.1	---	---	---	---	---
1,2,3-TRZ	420	570	540	0.84	433	411	0.041	---	10.0 +/− 0.4
1,2,4-TRZ	423	572	542	0.78	437	411	0.023 0.044	---	KD1=0.198±0.040 KD2 = 2.85 ± 0.82
IMZ	427	574	544	0.77	439	413	0.066	---	0.0989 +/− 0.0018

^*20°C

^#Maximum peak trough intensity normalized to 1 μM

^%Used as an estimate of efficiency of type II ligand to decrease inherent high spin enzyme fraction (type IIa)⁽²⁴⁾.