TABLE 5 .
siRNA(s) | No. of kan+ blue colonies sequenceda | No. with nucleotide insertedb |
Mutation frequency (%) | |||
---|---|---|---|---|---|---|
A | G | C | T | |||
NC | 190 (2) | 188 | 1 (5′ T) | 0 | 1 (3′ T) | 1.1 |
Polη | 142 (4) | 138 | 1 (5′ T), 2 (3′ T) |
0 | 1 (3′ T) | 2.8 |
Polκ | 192 (1) | 191 | 1 (3′ T) | 0 | 0 | 0.5 |
Rev3 | 186 (0) | 186 | 0 | 0 | 0 | 0 |
Rev7 | 190 (1) | 189 | 0 | 0 | 1 (3′ T) | 0.5 |
Polη + Polκ | 178 (1) | 177 | 1 (3′ T) | 0 | 0 | 0.6 |
Polη + Rev3 | 196 (2) | 194 | 1 (3′ T) | 0 | 1 (3′ T) | 1.0 |
Polκ + Rev3 | 194 (0) | 194 | 0 | 0 | 0 | 0 |
Numbers of mutant colonies are in parentheses.
The site where mutation occurred (the 3′ T or the 5′ T of the TT dimer) is shown in parentheses.