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. 2012 Sep 11;3(5):e00271-12. doi: 10.1128/mBio.00271-12

TABLE 5 .

Effects of TLS Pols on mutation frequencies and nucleotides inserted opposite a cis-syn TT dimer carried on the lagging-strand template of EBV plasmid in XPA human fibroblasts

siRNA(s) No. of kan+ blue colonies sequenceda No. with nucleotide insertedb
Mutation frequency (%)
A G C T
NC 190 (2) 188 1 (5′ T) 0 1 (3′ T) 1.1
Polη 142 (4) 138 1 (5′ T),
2 (3′ T)
0 1 (3′ T) 2.8
Polκ 192 (1) 191 1 (3′ T) 0 0 0.5
Rev3 186 (0) 186 0 0 0 0
Rev7 190 (1) 189 0 0 1 (3′ T) 0.5
Polη + Polκ 178 (1) 177 1 (3′ T) 0 0 0.6
Polη + Rev3 196 (2) 194 1 (3′ T) 0 1 (3′ T) 1.0
Polκ + Rev3 194 (0) 194 0 0 0 0
a

 Numbers of mutant colonies are in parentheses.

b

 The site where mutation occurred (the 3′ T or the 5′ T of the TT dimer) is shown in parentheses.