Table 2.

Comparative immunogenicity of wt fVIII, R372A/R1689A fVIII, and R372A/R1689A fVIII in fVIII^−/− mice

fVIII construct	Dose, μg	No. of ELISA titer positive	Mean/median ELISA titer	No. of inhibitor titer positive	Mean/median inhibitor titer, BU/mL
wt fVIII	0.2*	17/25	1490/400	13/25	44/10
	0.5	2/9	9/0	1/9	1/0
	1.0	5/9	100/11	3/9	14/0
	1.5	9/9	460/426	9/9	100/73
	2.0	10/10	1540/1760	10/10	310/310
R372A/R1689A fVIII	0.2*	10/25	470/0	6/25	4/0
	0.5	1/9	120/0	1/9	8/0
	1.0	5/9	170/16	3/9	29/0
	1.5	6/9	1170/74	6/9	160/8
	2.0	10/10	730/450	10/10	160/103
V634M fVIII	0.5	3/9	190/0	1/9	11/0
	1.0	5/9	140/10	4/9	16/0
	1.5	6/9	630/560	6/9	130/88
	2.0	9/10	2810/1480	9/10	450/290

^*P < .05 when compared with wt fVIII.