Figure 2.

Expanded views of the spectral region covering NOEs between base proton H8 and sugar protons H1′, H2′, and H3′ of monomeric ImpG in D₂O with 100 mM NaCl and 10 mM phosphate buffer (pH 7.8) at 4 °C. (A) NOESY spectrum (mixing time, 600 ms) of 10 mM ImpG in the absence of single-stranded template. (B) TrNOESY spectrum (mixing time, 100 ms) of 8 mM ImpG in the presence of an RNA single-stranded template (0.5 mM strand concentration). (C) TrNOESY spectrum (mixing time, 100 ms) of 9 mM ImpG in the presence of a DNA single-stranded template (0.6 mM strand concentration). The red cross-peaks in (A) correspond to positive NOEs having the opposite sign as the diagonal peaks; the black cross-peaks in (B) and (C) correspond to negative NOEs having the same sign as the diagonal peaks.