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. Author manuscript; available in PMC: 2012 Sep 21.
Published in final edited form as: Breast Cancer Res Treat. 2008 Dec 23;118(3):443–453. doi: 10.1007/s10549-008-0267-z

Table 1.

Details of antibodies and experimental conditions used for IHC

Biomarker Catalogue number Supplier Dilution Incubation Methoda
P-S104/6-ERα BL1636 Bethyl Labs, USA 1:200 1 h at 42°C CC1
P-S118-ERα 16J4 Cell Signaling, USA 1:600 1 h at 42°C CC1
P-S167-ERα 31478 Abeam, USA 1:700 1 h at 42°C CC1
P-S282-ERα BL16 Bethyl Labs, USA 1:700 1 h at 42°C CC1
P-S294-ERα BL16 Bethyl Labs, USA 1:800 1 h at 42°C CC1
P-S559-ERα BL16 Bethyl Labs, USA 1:150 1 h at 42°C CC1
P-T311-ERα BL1667 Bethyl Labs, USA 1:100 1 h at 42°C CC1
Total ERα 6F11 Novocastra, UK 1:50 1 h at 42°C CC1
a

Mild and standard cell conditioning, using CC1 antigen retrieval buffer (Ventana Medical Systems, AZ, USA)