Figure 2. Immune Responses of Vaccinated Mice.
Mice were primed intradermally with p36 (LACK) plasmid DNA (DNAp36; 100 μg) with or without α-galactosylceramide (2 μg αGalCer) and were boosted two weeks later with WRp36 (1×107 PFU) (as indicated in Figure 1). Shown are the results from ELISA analyses of splenic cells stimulated with either recombinant LACK antigen (p36) or soluble leishmanial antigen (SLA), three weeks after the final VVp36 boost:
, p36 antigen; □, soluble leishmanial antigen (SLA). These results are representative of 3 independent experiments.