Table 5. Immune Responses of Vaccinated and CD4 and CD8 Deficient/Depleted BALB/c Mice in Response to Leishmania major Infection.
| Group | IFNγ (ng/ml±SD) | Granzyme (ng/ml±SD) | IL-10 (pg/ml+SD) | Nitric Oxide |
|---|---|---|---|---|
| PBS (2×) | 0±0 | 0±0 | 981±60 | 0.7±0.2 |
| pCIneo+Galcer+WRluc | 0±0 | 0±0 | 822±37 | 1.5±0.5 |
| DNAp36+VVp36 | 66.2±1.2 | 1.5±0.3 | 282±15 | 15.6±0.3 |
| DNAp36+Galcer+VVp36 | 85.5±1 | 3.5±0.6 | 164±8 | 24±0.5 |
| DNAp36+VVp36 CD4dpl | 5.7±0.7 | 0.8±0.2 | 470±21 | 6.3±0.1 |
| DNAp36+VVp36 CD8dpl | 2.1±0.3 | 0.4±0.1 | 650±25 | 2.6±0.1 |
| DNAp36+Galcer+VVp36 CD4dpl | 7±0.8 | 1+0.1 | 343±14 | 8.6±0.2 |
| DNAp36+Galcer+VVp36 CD8dpl | 1.3±0.7 | 0.5±0.2 | 640±60 | 4±0.6 |
To examine the effects of in vivo CD4 and CD8 T cell depletion on the ongoing immune response to infection, cytokine responses were evaluated in vitro of splenic lymphocytes. Cells were treated or not with anti-CD4, or anti-CD8 as indicated in the Materials and Methods Section. Cells were then stimulated for 72 hours and the cytokine levels determined.