Table 1.

Plant genera for which flow cytometry has been used to measure the relative DNA content of extracted pollen nuclei

Genus	Family	No. of species	Primary methods	Tested methods	Reference
Alstroemeria	Alstroemeriaceae	1	C2, O2		6
Begonia	Begoniaceae	25*	O3, S	C1, O1, O2	4, 5
Brassica	Brassicaceae	1	S	C1, O1, O2	11
Cupressus	Cupressaceae	2	C2	Q	12
Dendranthema	Asteraceae	1	C1		2
Diospyros	Ebenaceae	4	C1		15, 16
Hibiscus	Malvaceae	2	C1		17
Lilium	Liliaceae	1†	C1, F		1, 2, 9, 18
Petunia	Solanaceae	1	C1		8
Rosa	Rosaceae	1 or 2‡	B, Q		7, 13
Rumex	Polygonaceae	2	C1, C3		3, 14
Triticum	Poaceae	1	C1		2
Tulipa	Liliaceae	2	F	C1?	10
Zea	Poaceae	1	C1		2

‘Primary methods’ are nuclei extraction methods used for main results; ‘Tested methods’ are extraction methods that were also tried. Methods: B = bead-beating, C = chopping (C₁ = simple chopping, C₂ = with germinated pollen, C₃ = frozen in buffer), F = filter bursting, O = osmotic bursting (O₁ = intact pollen, O₂ = with chemical/enzymatic protoplast release, O₃ = germinated pollen), S = sonication, Q = squashing. References: 1 = Akutsu et al. (2007), 2 = Bino et al. (1990), 3 = Błocka-Wandas et al. (2007), 4 = Dewitte et al. (2006), 5 = Dewitte et al. (2009), 6 = Hirano and Hoshino (2009), 7 = Jacob et al. (2001), 8 = Mishiba et al. (2000), 9 = Nukui et al. (2011), 10 = Okazaki et al. (2005), 11 = Pan et al. (2004), 12 = Pichot and El Maâtaoui (2000), 13 = Roberts (2007), 14 = Stehlik et al. (2007), 15 = Sugiura et al. (1998), 16 = Sugiura et al. (2000), 17 = Van Laere et al. (2009), 18 = Van Tuyl et al. (1989).

* Plus many hybrid cultivars.

^† Plus hybrid cultivars involving at least seven species.

^‡ Species is not stated in reference 7.