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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Clin Immunol. 2012 Aug 3;145(1):44–54. doi: 10.1016/j.clim.2012.07.010

Figure 2. Post trauma increased T cell apoptosis positively correlated with increased plasma Thrombospondin-1 levels.

Figure 2

(A) Patients experiencing elevated T cell apoptosis also had increased plasma Thrombospondin-1 levels. Elevated plasma TSP-1 levels in patients with increased T-cell apoptosis as determined by ELISA. T cell apoptosis was determined by assessing active Caspase-10 binding to FAM-AEVD-FMK peptide. *p<0.01 vs. control and immunocompetent Patients (Pt) T cells by Mann-Whitney U test compared to matched controls; n = 6 for each patient group. (B) Linear regression plot showing significant correlation between elevated plasma TSP-1 levels and T cell Caspase-10 activation. r2 = 0.734, **p < 0.001, n = 12. (C–D) Elevated T cell CD47 and PD-1 expression levels in patients who experienced T cell anergy. Freshly isolated T-cells from patients and matched controls were stained for CD47 (C) and PD-1 (D) by flowcytometry. Percent changes compared to parallely assessed T cells form matched controls. Dashed lines represent similar percent change values from immunocompetent patients’ T cells. All datapoints represent median value. *p<0.01 & #p < 0.05 by Mann Whitney U test, n = 8 for patients experiencing T cell anergy and 92 for patients’ immunocompetent T cells.