Skip to main content
. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Clin Immunol. 2012 Aug 3;145(1):44–54. doi: 10.1016/j.clim.2012.07.010

Figure 3. Stimulated control T-cells expressed increased active Caspase-10 following combined treatment with rhTRAIL & αCD47 antibody.

Figure 3

Freshly isolated T-cells were cultured with (i) 10μg/ml αCD Ab only, or (ii) immobilized αCD3 antibody alone, or (iii) αCD3+ αCD Ab, or (iv) αCD3 + rhTRAIL [1 μg/ml], or (v) αCD3 + rhTRAIL + αCD Ab for five days. Cleaved Caspase-10 levels were determined as described previously. Annexin-V binding levels were determined by flowcytometry using an AnnexinV + 7AAD kit. (A) Histograms showing active Caspase-10 levels in T-cells. (B) Graphical representation & statistics of above experiments *p < 0.01 by t test and ANOVA (n=8).