Table 2.
Immunohistochemical analysis of control and EL brain using anti-coxsackievirus antibody
| Dilution | 1:3,000 | 1:6,000 | 1:10,000 | 1:20,000 | 1:40,000 | 0 antibody |
|---|---|---|---|---|---|---|
|
Neutralizing dose/ml |
6.8 |
3.4 |
2 |
1 |
0.5 |
0 |
|
Controls, UK cases | ||||||
|
06/112 |
1+ |
|
2+ |
2+ |
0 |
|
|
06/110 |
2+ |
0 |
|
|
|
0 |
|
06/126/7 |
1+ |
|
|
|
|
|
|
05/152-9 |
1+ |
|
0 |
|
|
|
|
05/25- |
1+ |
|
|
|
|
0 |
|
05/47-11 |
0 |
|
|
|
|
|
|
NP86/07-4 |
0 |
|
|
|
|
|
|
05/137/5 |
1+ |
|
|
|
|
|
|
05/66-28 |
0 |
|
|
|
|
|
|
05-01-8 |
0 |
|
|
|
|
|
|
Controls, USA | ||||||
|
865183-M |
1+ |
1+ |
|
|
|
|
|
8889C |
2+ |
0 |
|
|
|
|
|
8854127-13 |
0 |
|
|
|
|
|
|
883599-11 |
2+ |
2+ |
2+ |
2+ |
|
|
|
1311056-17 |
1+ |
|
|
|
|
|
|
EL, USA Cases | ||||||
|
21225A |
|
3+ |
2+ |
2+ |
|
0 |
|
21225B |
|
2+ |
3.5+ |
2+ |
|
|
|
19940C |
4+ |
0.2+ |
0 |
|
|
0 |
|
19940B |
|
2.5+ |
4+ |
3+ |
0 |
0 |
|
91558 |
3+ |
2.5+ |
1+ |
|
|
1+ |
|
28065B |
0 |
1+ |
|
|
|
0 |
|
28065D |
3.5+ |
2.5+, 3+ |
3+, 3+ |
2+, 3+ |
|
0 |
|
28065C |
0.5+ |
3+, 3+ |
|
|
|
|
| 28065A | 3+ | 2+ | 0 | |||
Immunohistochemical analysis using anti-coxsackievirus B horse polyclonal antibody on brain from control and archival EL cases. The same expressions for quantitation are used as in Table 1. Case numbers followed by letters indicate different blocks from the same case. When tests were repeated, the two results are indicated in the same box.