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. 2012 Sep 21;7(9):e45077. doi: 10.1371/journal.pone.0045077

Figure 7. CYP1B1 mutants have lower 17β estradiol metabolizing activity compared to wild type protein.

Figure 7

A: The enzyme activity of the mutant proteins was expressed as percentage of the activity retained as compared to the native (wild type) enzyme. Mutant constructs of CYP1B1 (i.e. E229K, R368H and R523T) showed <10% of 17β estradiol metabolizing activity (**p-value<0.001). B : Histogram showing the expression level of the transfected wild type and mutant constructs of CYP1B1 in RPE cells as detected by RT-PCR. Three independent replicates were performed for this experiment.