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. 2012 Sep 21;7(9):e45077. doi: 10.1371/journal.pone.0045077

Figure 8. CYP1B1 mutants cause upregulation of MYOC in HTM cells.

Figure 8

A: Increased MYOC expression with mutant CYP1B1. Western blot analysis of mutant CYP1B1 and myocilin showed increased expression of MYOC in the presence of mutant CYP1B1 clones with reduced (<10%) 17β estradiol metabolizing activity. B : Quantitative analysis of MYOC expression. The histogram shows levels of expression of endogenous MYOC in HTM cells transfected with wild type and mutant CYP1B1 clones. All the three mutants of CYP1B1 (i.e. E229K, R368H and R523T) considerably over-expressed MYOC compared to the normal CYP1B1. The R368H and R523T showed statistically significant over expression of myocilin with a p-value of 0.023 and 0.014, respectively. However, the effect of E229K mutant was not found to be statistically significant. This experiment was repeated three times [*p-value- <0.05].