Table 3. Functional domain analysis.
| Gene | Function | Sites | SNPs | Prob. | pi | pi(known function) | Prob. |
| FET | pi(unassigned) | t-test | |||||
| Dcr1 | Unassigned | 39 | 0 | 0.0000 | |||
| DExD/H-like helicase | 474 | 11 | 0.0109 | ||||
| Unassigned | 972 | 50 | 0.0102 | ||||
| Helicase superfamily c-terminal domain | 327 | 9 | 0.0030 | ||||
| Unassigned | 465 | 32 | 0.0085 | ||||
| Double stranded RNA binding domain; pfam03368 | 288 | 10 | 0.0063 | ||||
| Unassigned | 513 | 9 | 0.0030 | ||||
| PAZ_dicer_like | 360 | 28 | 0.0070 | ||||
| Unassigned | 1524 | 39 | 0.0049 | ||||
| RIBOc1 | 255 | 3 | 0.0050 | ||||
| Unassigned | 633 | 35 | 0.0099 | 0.0063 | |||
| RIBOc2 | 333 | 19 | 0.9448 | 0.0056 | 0.0061 | 0.9109 | |
| Dcr2 | Unassigned | 63 | 6 | 0.0126 | |||
| DExD/H-like helicase | 426 | 28 | 0.0054 | ||||
| Unassigned | 519 | 14 | 0.0048 | ||||
| HELICc | 321 | 13 | 0.0029 | ||||
| Unassigned | 208 | 24 | 0.0074 | ||||
| Double stranded RNA binding domain; pfam03368 | 266 | 10 | 0.0046 | ||||
| Unassigned | 483 | 23 | 0.0081 | ||||
| PAZ | 351 | 13 | 0.0058 | ||||
| Unassigned | 684 | 25 | 0.0055 | ||||
| RIBOc1 | 246 | 3 | 0.0006 | ||||
| Unassigned | 465 | 12 | 0.0042 | ||||
| RIBOc2 | 485 | 15 | 0.0046 | 0.0040 | |||
| Unassigned | 229 | 8 | 0.6061 | 0.0024 | 0.0064 | 0.1322 | |
| Ago1 | Domain of Unknown Function DUF1785; Pfam 08699 | 159 | 0 | 0.0000 | |||
| PAZ_argonaute_like | 273 | 3 | 0.0015 | ||||
| Piwi_ago-like | 1257 | 62 | 0.0081 | ||||
| Piwi domain; pfam02171 | 885 | 41 | 0.0088 | ||||
| 5′ RNA guide strand anchoring site | 12 | 1 | 0.0008 | 0.0038 | |||
| Unassigned | 204 | 8 | 1.0000 | 0.0093 | 0.0093 | - | |
| Ago2 | Unassigned | 933 | 22 | 0.0059 | |||
| Domain of Unknown Function DUF1785; Pfam 08699 | 147 | 6 | 0.0087 | ||||
| PAZ_argonaute_like | 312 | 12 | 0.0069 | ||||
| Unassigned | 513 | 18 | 0.0081 | ||||
| Piwi domain; pfam02171 | 912 | 14 | 0.0027 | 0.0061 | |||
| Unassigned | 78 | 2 | 0.1482 | 0.0014 | 0.0052 | 0.7332 | |
| R3D1 | Unassigned | 195 | 4 | 0.0036 | |||
| Double-stranded RNA binding motif | 135 | 3 | 0.0069 | ||||
| Unassigned | 138 | 6 | 0.0010 | ||||
| Double-stranded RNA binding motif | 201 | 8 | 0.0061 | ||||
| Unassigned | 96 | 2 | 0.0030 | ||||
| Double-stranded RNA binding motif | 198 | 6 | 0.0061 | 0.0063 | |||
| Unassigned | 15 | 0 | 0.7084 | 0.0000 | 0.0019 | 0.0099 | |
| R2D2 | Unassigned | 30 | 2 | 0.0010 | |||
| Double-stranded RNA binding motif | 210 | 4 | 0.0009 | ||||
| Unassigned | 66 | 3 | 0.0155 | ||||
| Double-stranded RNA binding motif | 153 | 1 | 0.0014 | 0.0011 | |||
| Unassigned | 441 | 11 | 0.1775 | 0.0064 | 0.0076 | 0.2665 |
The proportions of segregating sites in regions of known function versus regions where functions have not been assigned were compared by Fisher's Exact Test (FET) and the resulting probability is listed. Average values of pi(average number of nucleotide differences per site between sequence pairs) were compared between regions of known versus unassigned function using a student's t-test and a significant difference was only seen for R3D1, in which pi was greater in regions of known function.