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. 2012 Sep 21;7(9):e43291. doi: 10.1371/journal.pone.0043291

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C) FAK+/+ osteoblasts form focal adhesions (red) as shown by vinculin staining and display prominent actin fiber formation (green) as shown by phalloidin staining. (D–F) FAK−/− osteoblasts also exhibit actin fiber and focal adhesion formation. Panels C and F show merged images. DAPI nuclei stain in blue. Magnification = 60×. Scale bar represents 25 µm.