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. 2012 Sep 21;7(9):e43291. doi: 10.1371/journal.pone.0043291

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A–C) Paxillin expression in MC3T3 osteoblasts, FAK+/+ osteoblasts and FAK−/− osteoblasts is similar. (D–F) Overlay of paxillin (red) and F-actin (green) as shown by phalloidin staining shows regions of strong paxillin expression and localization corresponding to the termini of actin fibers. FAK−/− cells exhibited fewer numbers of focal adhesions per cell area as quantified by point counting. DAPI nuclei stain in blue. Magnification = 60×. Scale bar represents 25 µm.