Table 1. Oligonucleotide primers for mRNA quantification by quantitative real-time reverse transcription-PCR.
mRNA | Forward primer 5′-3′ | Reverse primer 5′-3′ |
OSBP | GATCCATCAGGAAAAGTCCAC | CAGTGCCACTTTCCCAAGCA |
ORP1S | GGTCCTCGGATCTGGCCCA | ACTCAGGGACCTTTCGGACTC |
ORP1L | TGATTGCCTTAATCTCTTCACC | ACTCAGGGACCTTTCGGACTC |
ORP2 | ACCACCTGAGAAAGGCCAAGC | CTCCAGCTCGTTGAGGCTCAC |
ORP3 | GCATCTAGCTACTACCGAGCT | CACATGGGTTGTGCCAATTGGA |
ORP4 | AAGCTCTGGATCGACCAGTCA | CTGGGACTGCTATGCATGACC |
ORP5 | GTGCCGCTGGAGGAGCAGAC | AGGGGCTGTGGTCCTCGTATC |
ORP6 | GGATACTGCTCCACCTATTTCA | ACAGGCAGGATTTCCATCGAC |
ORP7 | CGGCCTACTCCTCCACATACC | ACTGTTCCCACAGGCACAATC |
ORP8 | GAAGAACAGGGAGATTTTGAATCA | TCCTGTGAGTGGATCAAGTTC |
ORP9 | CATCTTCCACACTAAACCCTTC | CTCGTTCTGATCTTCCAACTTC |
ORP10 | CTCAGCGACAGTGATATTCCAC | AGGTCTGATCTTCTTGGGATAC |
ORP11 | CACATTTTCTCTACCCTGTGCA | CCCTTGCACTCTGCATACCAC |
Adiponectin | GGCCGTGATGGCAGAGAT | CCTTCAGCCCGGGTAC |
aP2 | GCTTTTGTAGGTACCTGGAAACTT | ACACTGATGATCATGTTAGGTTTGG |
Leptin | GCCCTATCTTTTCTATGTCC | TCTGTGGAGTAGCCTGAAG |
PPARγ | GATCCAGTGGTTGCAGATTACAA | GAGGGAGTTGGAAGGCTCTTC |
SDHA | CATGCTGCCGTGTTCCGTGTGGG | GGACAGGGTGTGCTTCCTCCAGTGCTCC |
β-actin | GACAGGATGCAGAAGGAGATT | TGATCCACATCTGCTGGAAGG |