Figure 4. Phylogenetic tree of Dsr-LP and Dsr based on Maximum Likelihood method.
Dsr-LP (Groups Ia-d and IIIa-d) and Dsr, defined in Fig. 3 and its legend. Dsr and Fsr-C, defined in the legend of Fig. 1. Dv-DsrA/B and Af-DsrA/B, Dsr subunits A and B of Desulfovibrio vulgaris strain Hildenborough (ORFs DVU0402 and DVU0403) and Archaeoglobus fulgidus DSM 4304 (ORFs AF0423 and AF0424), respectively [64], [65]; Dv-alSir and CPE1438, anaerobic small sulfite reductase of Desulfovibrio vulgaris strain Hildenborough (ORF DVU_1597) and Clostridium perfringens strain 13, respectively [66]; The ORF numbers followed by “-C”, Fsr-C homologs. Abbreviation for organism names preceding the listed ORF numbers: MTBMA, Methanothermobacter marburgensis strain Marburg; MTH, Methanothermobacter thermautotrophicus ΔH; Metbo, Mehanobacterium sp. AL-21; RMTH, Methanothermococcus thermolithotrophicus (sequence obtained from Dr. William B. Whitman, University of Georgia); Mfer, Methanothermus fervidus DSM 2088; Maeo, Methanococcus aeolicus Nankai-3; Msp, Methanosphaera stadtmanae DSM 3091; MMP, Methanococcus maripaludis S2; Mpal, Methanosphaerula palustris E1-9c; Mpet, Methanoplanus petrolearius DSM 11571; Memar, Methanoculleus marisnigri JR1; MM, Methanosarcina mazei Gö1; MA, Methanosarcina acetivorans C2A; Mbar, Methanosarcina barkeri strain Fusaro; Mbur, Methanococcoides burtonii DSM 6242; Mmah, Methanohalophilus mahii DSM 5219; Metev, Methanohalobium evestigatum Z-7303; Mthe, Methanosaeta thermophila PT; MCON, Methanosaeta concilii GP-6; MCP, Methanocella paludicola SANAE; Mboo, Candidiatus Methanoregula boonei 6A8; Mlab, Methanocorpusculum labreanum Z; Mhun, Methanospirillum hungatei JF-1; Metbo, Methanobacterium sp. Al-21; Metok, Methanothermococcus okinawensis IH1; Metin, Methanocaldococcus infernus ME; MFS40622, Methanocaldococcus sp. FS406-22; MJ, Methanocaldococcus jannaschii DSM 2661; Mefer, Methanocaldococcus fervens AG86; Metvu, Methanocaldococcus vulcanius M7; MK, Methanopyrus kandleri AV19; GZ27A8_52, uncultured archaeon related to Methanosarcina and a member of an anaerobic methane oxidizing consortium; RCIX2692 and RCIX2197, uncultured methanogenic archaeon RC-1 and primary methane producer in rice rhizosphere. The bootstrap value shown at each branch is from 1000 replicates. Scale bar, number of amino acid substitutions per site. *, shows outliers. A and B, sub-clades of group IIId.