Table 2. Examples of successful expression construct optimization.
Modification | Host | Target protein | rpa improvement | Reference |
Promoter | Tobacco protoplasts | GUS | 4-fold | [35] |
Leader sequence | Tobacco | GUS A | 8.6-fold | [36] |
Scaffold attachment region | Tobacco suspension culture | GUS | 12-fold | [37] |
Localization | Tobacco | LT-B* | 1.2–2.8-fold | [38] |
Fusion protein | Tobacco | GFP | 2-fold | [39] |
Localization | Tobacco | Antibody 14D9 | 2-fold | [40] |
This table gives several examples for which the accumulation level of the target protein was improved by modifying the expression strategy.
E. coli heat-labile enterotoxin B subunit (LT-B).