Table 2. Examples of successful expression construct optimization.

Modification	Host	Target protein	rpa improvement	Reference
Promoter	Tobacco protoplasts	GUS	4-fold	[35]
Leader sequence	Tobacco	GUS A	8.6-fold	[36]
Scaffold attachment region	Tobacco suspension culture	GUS	12-fold	[37]
Localization	Tobacco	LT-B*	1.2–2.8-fold	[38]
Fusion protein	Tobacco	GFP	2-fold	[39]
Localization	Tobacco	Antibody 14D9	2-fold	[40]

This table gives several examples for which the accumulation level of the target protein was improved by modifying the expression strategy.

^* E. coli heat-labile enterotoxin B subunit (LT-B).