Table 5. Construct screening.

	Apoplast	ER	Vacuole
DsRed [µg/mg TSP]	2.1±1.0	1.3±0.3	16.8±7.8
Phl p 1 [µg/mg TSP]	4.1±1.8	3.3±2.1	41.6±21.0
M12 [µg/mg TSP]	43.9±3.6	14.9±10.6	−

Three sets of 12 leaf discs each were infiltrated using Agrobacterium containing a construct coding for one of the eight constructs. After infiltration and a five-day incubation period (25°C, 16/8 h light/dark cycle, 7 klux), nine leaf discs from each set were pooled and extracted simultaneously. For phl p 1 and M12, the recombinant protein concentrations were determined via ELISA. The DsRed concentration was determined by measuring the fluorescence. All values were normalized to the TSP content as determined via Bradford assay. The max/min ratio indicates the difference between the highest and lowest concentration measured for each model protein.