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. 2012 Sep 21;7(9):e45741. doi: 10.1371/journal.pone.0045741

Figure 6. Rhomboid-encoding genes from MTB and MAC complement MSMEG_4904 and MSMEG_5036.

Figure 6

Panel 1: shows the Δ4904 single mutant (Δ4904) not growing on solid medium with 0.1 µg ml−1 ciprofloxacin while the wild type and the double mutant (Δ4904Δ5036) grow. Panel 2: When transformed into the double mutant (Δ4904Δ5036), plasmids with the gene encoding Rv0110 (orthologous to MSMEG_5036) from MTB produced a phenotype of the Δ4904 single mutant (labeled Δ4904Δ5036+Rv0110). Similarly, transforming the single mutant with a plasmid encoding MAV_1554 (orthologus to MSMEG_4904) of M. avium also restored growth of the Δ4904 single mutant on 7H10 with 0.1 µg/ml ciprofloxacin (labeled Δ4904+MAV_1554). Panel 3: Transforming the Δ4904 single mutant with a plasmid encoding Rv1337 (orthologous to MSMEG_4904) of MTB restored its growth on 7H10 with 0.1 µg/ml ciprofloxacin (labeled Δ4904+Rv1337). Similarly, transforming the Δ4904 single mutant with a plasmid encoding MSMEG_4904 restores its growth on 7H10 with 0.1 µg/ml ciprofloxacin (labeled Δ4904+MSMEG_4904). Panel 4: Transforming the Δ4904 single mutant with a plasmid containing both genes encoding MAP2426c and MAP2425c (orthologous to MSMEG_4904) restored its growth (albeit weakly) (labeled Δ4904+MAP2425c/2426c).