Table 1. Effect of ex vivo T cell phenotype on susceptibility of unstimulated PBMC to infection with HIVML1956 virus at a MOI of 0.1.
| T cell phenotype | No Detectable Virus (n = 7) | Virus Detected (n = 14) | p-valuea |
| Relative Expressionb | |||
| CD4+ CD69+ | 0.41 (0.24–1.00) | 1.45 (0.48–2.31) | 0.028 |
| CD4+ CD38+ | 38.8 (23.8–49.6) | 42.3 (34.9–46.2) | 0.628 |
| CD4+ HLA DR+ | 1.56 (0.80–2.32) | 1.65 (1.24–2.48) | 0.479 |
| CD4+ CD38+/HLA DR+ | 0.75 (0.28–1.20) | 0.73 (0.61–1.24) | 0.737 |
| CD4+ CCR5+ | 0.70 (0.46–1.56) | 0.99 (0.63–1.35) | 0.628 |
| CD4+ CXCR4+ | 73.6 (35.2–86.1) | 54.0 (40.8–84.0) | 0.970 |
| CD8+ CD69+ | 1.06 (0.53–1.88) | 1.91 (0.67–4.59) | 0.192 |
| CD8+ CD38+ | 47.2 (41.1–48.2) | 44.9 (43.3–46.8) | 0.852 |
| CD8+ HLA DR+ | 4.90 (2.92–8.60) | 4.52 (3.56–5.28) | 0.794 |
| CD8+ CD38+/HLA DR+ | 2.81 (1.58–4.50) | 2.44 (1.92–2.74) | 0.682 |
| Tregs (CD25+ FOXP3+) | 1.07 (1.00–1.43) | 0.94 (0.78–1.12) | 0.093 |
| Treg/CTLA-4+ | 23.2 (11.1–26.4) | 22.0 (18.9–27.7) | 0.682 |
| Estimated number of cellsc | |||
| CD4+ CD69+ | 314.7 (156.5–432.5) | 677.6 (311.4–1150) | 0.093 |
| CD4+ CD38+ | 19195 (15565–35354) | 24400 (14117–29307) | 0.970 |
| CD4+ HLA DR+ | 804.6 (520.9–1212) | 1020 (663.4–1335) | 0.737 |
| CD4+ CD38+/HLA DR+ | 476.1 (182.6–585.1) | 476.1 (281.0–688.7) | 0.794 |
| CD4+ CCR5+ | 452.8 (206.1–1195) | 482.4 (344.7–881.0) | 0.970 |
| CD4+ CXCR4+ | 47987 (15983–59581) | 31633 (18104–51924) | 0.576 |
| CD8+ CD69+ | 210.9 (109.2–332.8) | 379.3 (183.3–1013) | 0.093 |
| CD8+ CD38+ | 8514 (7642–12602) | 10586 (7726–13124) | 0.433 |
| CD8+ HLA DR+ | 779.6 (499.8–2236) | 1139 (798.6–1341) | 0.852 |
| CD8+ CD38+/HLA DR+ | 563.4 (286.6–1160) | 615.7 (450.7–743.0) | 0.970 |
| Tregs (CD25+ FOXP3+) | 740.4 (393.4–972.8) | 537.6 (347.2–717.4) | 0.109 |
| Treg/CTLA-4+ | 171.8 (42.66–285.3) | 107.0 (62.77–222.3) | 0.433 |
a
p-values calculated using non-parametric Mann-Whitney U test. Parameters that demonstrated statistically significant (p<0.05) or trending (p<0.10) p-values are highlighted in bold text.
b
Relative expression of phenotypic markers is listed as median of percent positive (+) cells. Interquartile range is shown in parentheses.
c
Estimated number of cells was calculated from flow cytometry proportions based on seeding 105 cells per well. Interquartile range is shown in parentheses.