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. 2012 Sep 21;7(9):e45858. doi: 10.1371/journal.pone.0045858

Figure 2. Arhgef15 mediates VEGF-induced Cdc42 activation and potentiates RhoJ inactivation.

Figure 2

(A) Ectopic expression of full-length Arhgef15 (GEF15) and its DH-PH truncated proteins activated endogenous Cdc42 in 293T cells. (B) VEGF-induced Cdc42 activation at 5 min was abolished by Arhgef15 knockdown in HUVECs. (C) Ectopic expression of GEF15 and its DH-PH proteins inactivated co-transfected RhoJ-WT in 293T cells. CA, constitutively-active; DN, dominant negative. In A–C, representative immunoblotting images of 3 independent experiments are shown. Error bars represent SEM; *P<0.05, **P<0.01. (D) A scheme xrepresenting signal transduction in ECs.