Multiple alignments of selected RasGEFN domains.
A conserved region was found in the N-terminal regions of several
proteins with RasGEF (Cdc25-like) domains (37). Surprisingly, this
N-terminal domain may be present in the sequence either close to, of
far from, the RasGEF domain. A psi-blast search using a
region (residues 898–946) of C. albicans Cdc25
(CC25 CANAL) and E < 0.01, identified each of the
sequences in Fig. 3 within nine passes before convergence. Predicted
(54) secondary structure and 90% consensus sequences are shown beneath
the alignments; SwissProt/PIR/EMBL accession codes and
residue limits are given after the alignments. Residues are colored
according to the consensus sequence [green: hydrophobic (h),
ACFGHIKLMRTVWY; blue: polar (p), CDEHKNQRST; red: small (s), ACDGNPSTV;
red: tiny (u), AGS; cyan: turn-like (t), ACDEGHKNQRST; green: aliphatic
(l), ILV; and, magenta: alcohol (o), ST). The SwissProt sequence
KMHC DICDI has been altered to account for probable frameshifts.