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. 2012 Sep 21;7(9):e46121. doi: 10.1371/journal.pone.0046121

Figure 1. Schematic linear representation of the expression plasmids and SDS-PAGE analysis of Cry11A, Cry4Aa and Cyt2Ba expression.

Figure 1

Panels A, B and C: cyt2BaHis, cry4Aa and cry11A genes inserted, respectively, into the BamHI cloning site of the pSVP27A plasmid. The recombinant genes are under the control of a sporulation phase promoter, Pcyt. The genes for positive clone selection in E. coli (Amp-r) and in B. thuringiensis (CAT-r) are shown. Selected restriction enzyme sites used to confirm cloning are also shown. Panels D and E: SDS-PAGE of purified protoxins (Cry11A, Cry4Aa and Cyt2Ba) from recombinant B. thuringiensis strains. As a control, a sporulated acrystaliferous B. thuringiensis strain (4Q7) extract was used. Selected molecular masses of the protein markers are shown (ColorPlus™ Prestained Marker-NEB, panel D and BenchMark™ Protein Ladder-Invitrogen, Panel E) on the left side of both panels.