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. 2012 Oct;26(10):4230–4242. doi: 10.1096/fj.12-207969

Figure 1.

Figure 1.

Actn2-GFP recapitulates the localization of endogenous Actn2 and assembles early into the Z disc. A) In Tg(ttna:actn2-egfp) fish, a ttna enhancer drives the expression of EGFP-tagged Actn2 in the somites starting at 5 S and in the heart starting at 18 S. EGFP signal can be detected in both the heart and somites at 48 hpf. H, heart; B) Images of dissected hearts or somites from Tg(ttna:actn2-egfp) fish. Actn2-egfp is localized to Z bodies as irregular dots (empty arrowhead) at 18 S and periodic Z discs (arrow) at 48 hpf in the heart. In the somites, Actn2-egfp forms Z bodies as regular dots (solid arrowhead) at 18 S and striated Z discs (arrow) at 21 S. C) Left panel: examples of 3 types of CMs exhibiting different striation that may coexist in embryos injected with constructs encoding EGFP-tagged Z-disc proteins. Right panel: quantification of the percentage of CMs that exhibit different degrees of striation at 48 hpf. Compared to MYOTILIN-EGFP, Tcapa-EGFP, and Mlp-EGFP, injection of Actn2-EGFP resulted in a higher percentage of CMs exhibiting clear striation. n, CM number. Scale bars = 10 μm (B); 5 μm (C).