Skip to main content
. 2012 Oct;26(10):4230–4242. doi: 10.1096/fj.12-207969

Figure 4.

Figure 4.

Depletion of Actn2 leads to lateral alignment defects in the Z discs. A) a–c) Electron micrographs of CMs of 52-hpf embryos at low magnification. Compared to WT fish, actn2 morphants exhibit organized myofibrils but disrupted Z-disc structures (arrows). d–f) High-magnification images of boxed regions panels a–c. In contrast to the laterally aligned Z disc in WT fish (d), the electron-dense Z discs in actn2 morphants are misaligned, exhibiting as either zigzagged lines (e) or dispersed dots (f). Arrowheads indicate dispersed electron-dense dots. Brackets indicate Z-disc width. Scale bars =1 μm (a–c); 200 nm (d–f). B) Measurement of Z-disc width based on electron microscopy data. C) Schematic of the Z-disc defects in actn2 morphants. Injection of actn2 MO blocks the lateral alignment but not the lateral fusion of the Z discs. MO, fish injected with actn2 MO. *P < 0.05.