Figure 1.

Effective knockdown of SPL expression in MIN6 cells by using siRNA technology. A) Representative Western blot indicating SPL expression after treatment of MIN6 cells with 2 different SPL siRNAs (SPL-1 and SPL-2) and 2 different scrambled control siRNAs (Con-1 and Con-2). For control purposes, brain lysates from SPL^−/− mice and WT littermates were also included in this analysis (first 2 lanes). Forty micrograms of total protein was loaded per well. B) Quantification of immunoblotting results. SPL protein expression levels are expressed relative to those found with cells treated with Con-1 siRNA (=100%). Data are given as means ± se of 4 independent experiments. **P < 0.01 vs. Con-2 siRNA-treated cells.