Figure 2.

Knockdown of SPL expression in MIN6 cells results in enhanced M3R-mediated increases in [Ca²⁺]_i. MIN6 cells were electroporated with 2 different scrambled control siRNAs (Con-1 and Con-2) and 2 different SPL siRNAs (SPL-1 and SPL-2). A) Representative concentration-response curves for muscarinic agonist OXO-M-induced increases in [Ca²⁺]_i. B) Summary of basal and maximum agonist-stimulated [Ca²⁺]_i levels derived from OXO-M concentration-response curves. AU, arbitrary units. Data are expressed as means ± se of 4 independent experiments, each carried out in duplicate. *P < 0.05 vs. Con-1 siRNA-treated cells.