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. Author manuscript; available in PMC: 2013 Sep 11.
Published in final edited form as: Biochemistry. 2012 Aug 29;51(36):7189–7201. doi: 10.1021/bi300865g

Figure 1.

Figure 1

Examples of ITC experiments showing the binding of p53, TRTK12, NDR, HDM2, and HDM4 peptides to S100B in the presence of calcium. Plots represent the raw heat effects (μJ/sec) as a function of time, the cumulative heat effects (kJ/mol, represented by closed circles) as a function of the molar ratio of peptide to protein, and the fits to the experimental data (solid lines) for: p53 binding to S100B at 25 °C (Panel A; data fitted to equation 2), TRTK12 binding to S100B at 25 °C (Panel B; data fitted to equation 2), NDR binding to S100B at 30 °C (Panel C; data fitted to equation 3), HDM2 binding to S100B at 35 °C (Panel D; data fitted to equation 2), HDM4 binding to S100B at 10 °C (Panel E; data fitted to equation 2), HDM2 binding to S100B at 15 °C (Panel E; data fitted to equation 1).