CV-N differentially recognizes various mycobacterial species. A, M. tuberculosis strain mc26020, M. bovis BCG strain Copenhagen, M. smegmatis strain mc2155, and E. coli strain DH5α were coated on a 96-well plate and probed with a serial dilution of CV-N+HRP. CV-N binding was detected using OPD as coloring reagent and absorption was measured at 490 nm. B, In a similar assay, binding of CV-N to M. tuberculosis strains H37Rv, H37Ra, CDC1551, and HN878 was tested. In all cases, the data represent the average of three independent experiments plus the standard error of mean.