FIGURE 6.

Impaired Th1 immunity and Th17 skewing in P. gingivalis-infected TLR4-deficient mice. Expression of IFN-γ and IL-17 in (A) CD4⁺ and (B) CD8⁺ splenic T cells from uninfected and P. gingivalis-infected mice after in vitro stimulation with 10 μg/ml P. gingivalis soluble Ags and 1 μg/ml anti-mouse CD28 for 4 h in the continuous presence of brefeldin A. Data represent mean ± SD, n = 4/group. White bar, uninfected; gray bar, P. gingivalis infected. Intragenotype comparisons were calculated by Mann–Whitney U test. **p < 0.01, ***p < 0.001. Intergenotype comparisons were calculated by two-way ANOVA with Bonferroni’s posttest. (C and D) Representative flow cytometry histogram analysis of cytokine-secreting CD4⁺ and CD8⁺ T cells (filled histogram is isotype control).