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Th1 and Treg polarizing cytokine production after P. gingivalis infection is impaired in DCs from TLR4-deficient mice. Bone marrow-derived DCs from C57BL/6 (white bars) and TLR4−/− (gray bars) mice were infected with P. gingivalis at the indicated multiplicity of infection (MOI). After 24 h, supernatants were analyzed for (A) IL-12, (B) IL-10, and (C) IL-6 by ELISA. Bars represent mean ± SD from triplicate cultures. **p < 0.01, ***p < 0.001 (Student t test).
